Vladimir was hired by the Weyland Corporation to provide security aboard the USCSS Prometheus during its voyage to the distant moon LV-223. After spending two years in hypersleep, Vladimir along with the other crew members were awaken. As the Prometheus nearly arrives on LV-223, Vickers, Shaw and Holloway briefed the crew on their mission to find the Engineers. After the Prometheus crew's first expedition to the Engineer temple, he helped David retrieve Holloway and Shaw after they are trapped by the Silica storm outside the Prometheus. He stood by one of the ship's airlocks helping Holloway and Shaw board the vessel when David finally rescues them.
Vladimir along with fellow mercenaries Jackson, Sheppard and Taplow later attempted to kill Fifield who has been mutated by the Black Liquid and was running rampant at their ship's hangar bay by shooting him multiple times with pistols. When the guns prove ineffective to kill it he tries to protect himself inside the RT-01 Transport, but is hit in his back with an axe by Fifield and pulled out of the vehicle, instantly killing him.