Bishop was a Hyperdyne Systems model 341-B Synthetic technician with the United States Colonial Marine Corps. He was part of a search and rescue mission sent to LV-426 aboard the USS Sephora in 2179 to investigate the disappearance of the USS Sulaco and its crew 17 weeks earlier. For all intents and purposes, he is identical to the Bishop who was stationed aboard the Sulaco.
- Bishop is voiced by and modelled after actor Lance Henriksen, who portrayed Lance Bishop in Aliens, Michael Bishop in Alien3 and Charles Bishop Weyland in Alien vs. Predator, and also lent his voice and likeness to the character of Karl Bishop Weyland in the video game Aliens vs. Predator.
- Furthermore, Henriksen appeared as Detective Hal Vukovich in The Terminator, which was written and directed by Aliens director James Cameron. In the film, his character is killed by the titular cyborg; this, combined with his deaths in Aliens and Alien vs. Predator, makes Henriksen one of two actors who have been killed on-screen by an Alien, a Predator and a Terminator, a dubious honor he shares with fellow Aliens actor Bill Paxton.
- The Sephora Bishop is the first canon instance in the Alien franchise where multiple versions of the same android model have been shown. In fact, Hicks mistakes Bishop for the android from the USS Sulaco when they first meet on LV-426, leading the former android to point out he is a "different Bishop".
- Prior to the game's release, many sources attributed the name "Rook" to the character. Rook is a piece found in the game of chess, as is a Bishop. "Rook" was also the nickname of Friedric Marcus, a character from the video game Aliens: Infestation, which was also published by Gearbox Software which had a very similar plot to Aliens: Colonial Marines. The game even features Marines from a ship called the USS Sephora investigating the USS Sulaco (like in Aliens: Colonial Marines).
- Aliens: Colonial Marines (video game)