- "Please understand, this is our best shot. Someone's got to re-establish comms."
- ―Hughes (from Alien: Isolation)
Hughes was a resident of Sevastopol Station, along with his wife Marie, daughter Claire and their cat. When a lone Xenomorph was unleashed on the station by the crew of the Anesidora in 2137, he became one of the survivors desperately waiting for rescue. He was encountered by Amanda Ripley when she arrived on Sevastopol to recover the USCSS Nostromo's flight recorder, which had also been brought there by the Anesidora.
Hughes was seen arguing with a Working Joe who was positioned to defend the elevator to Seegson Communications. He attempted to enter the elevator but was caught by the synthetic. An argument ensued which eventually led to the Joe lunging for Hughes, forcing him to shoot the Working Joe. In response, the Joe grabbed Hughes and slammed him into a wall. While Hughes tries to stand back up, the Joe grabs him a second time and repeatedly bashes his skull against the wall until he dies, splattering his blood all over the wall. Amanda looks on in horror as the Joe ends the confliction with a simple "good day" and walks off.
A few audio logs recorded by Hughes can be picked up in the station, as well as some that mention him.
- Hughes is a playable character in Alien: Isolation DLC Safe Haven.
- An Easter egg in Alien Isolation is located in the Sevastopol Spaceflight Terminal where the player, before encountering Axel, can go through a door located to the right. If the player listens carefully, they can hear a meowing cat. The cat eventually let's out a screech and is silenced, possibly indicating that the Drone had gotten to it. This may have possibly been Hughes's cat.
- The scene in which Hughes is killed is known as "BensonScene" within the game's code. This could indicate that Hughes' original name was "Benson".