- "Um, Miss Vickers, is there an agenda that you're not telling us about?"
- ―Holloway (from Prometheus)
Charles "Charlie" Holloway was an archaeologist who was assigned to assist the USCSS Prometheus expedition in discovering the homeworld of the Engineers on LV-223. He has a romantic relationship with Elizabeth Shaw.
An archeologist, he believes more in science than religion. Charlie is in a romantic relationship with Elizabeth Shaw, a fellow scientist and archeologist. Over the course of the film Holloway is infected with the Engineer Black Liquid by David. The infection at first very slowly mutates him and no symptoms are present. Holloway eventually has sex with Shaw and unknowingly impregnates her with an alien monster.
Later, the infection spreads more and causes him a severe feverish state and intense pain afterwards. Other symptoms then start to show, such as bloodshot eyes, very visible dark veins, rapidly deteriorating skin tissue and a very pale, almost livid skin color.
To save the life of his beloved Elizabeth and to stop his pain, Holloway is burned alive at his own request by a reluctant Vickers, who refused to let him back on board since he could contaminate and infect other crewmen.
- Charles Holloway was originally named Martin Holloway in the early script by Jon Spaihts. In that script, Holloway is impregnated by an Octo-Facehugger that comes from an egg in the ceiling of a chamber in the pyramid, he is later killed by the Beluga-Xenomorph Chestburster erupting from him.